
Biological Clocks &
Circadian Rhythms
Time-Restrictive Feeding (TRF) and Biological Clocks
2024Fall - 2025Fall
Research Hypothesis

Analysis Rationale
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We broke these 12 people into two experimental groups:
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Group 1: Feeding was allowed only from 8 am - 6 pm
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Group 2: Feeding was allowed only from 10 am - 8 pm
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Before beginning the experiment all participants filled out the Pittsburgh Sleep Quality Index (PSQI)
In a population of college students time-restricted feeding parameters will improve sleep quality and increase duration. When students cease eating at either 6 pm or 8 pm, their bodies will not be actively metabolizing during their sleep, allowing sleep rhythms to normalize.
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Late night snacking forces your body to metabolize during hours when it would normally not do so
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This can cause weight gain, in addition to sleep disruption
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Late night snacking may also interfere with your body’s sleep processes, including Process S and Process C

Data Analysis
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Goal: Determine whether feeding windows influenced sleep quality and duration
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Objective and subjective measures
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Utilized PRISM to analyze raw data

Result
Statistically speaking, the sleep duration and qualities of both experimental groups are not significantly different from the control group.

Discussion
Insulin spike after food ingestion might act as a neuropeptide in the central nervous system and change sleep pattern
TRF---↓insulin signaling---↓sleep amount